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A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG BJM
Assaf,Jean Claude; Atoui,Ali; Khoury,André El; Chokr,Ali; Louka,Nicolas.
ABSTRACT Several strains of lactic acid bacteria (LAB), frequently used in food fermentation and preservation, have been reported to bind different types of toxins in liquid media. This study was carried out to investigate the effect of different concentrations of Lactobacillus rhamnosus GG (ATCC 53103) to bind aflatoxin M1 (AFM1) in liquid media. AFM1 binding was tested following repetitive washes or filtration procedures in combination with additional treatments such as heating, pipetting, and centrifugation. The mixture of L. rhamnosus GG and AFM1 was incubated for 18 h at 37 °C and the binding efficiency was determined by quantifying the unbound AFM1 using HPLC. The stability of the complexes viable bacteria-AFM1 and heat treated bacteria-AFM1 was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin M1; Lactic acid bacteria; L. rhamnosus GG; Milk.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100120
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A survey of whole-plant corn silages from Minas Gerais dairy farms Scientia Agricola
Santos,Andréia de Oliveira dos; Dias Junior,Gilson Sebastião; Pereira,Marcos Neves; Schwan,Rosane Freitas; Ávila,Carla Luiza da Silva.
ABSTRACT Whole-plant corn silage (WPCS) is a commonly used forage for feeding dairy cattle in Brazil. The aim of this study was to evaluate on-farm ensilaging practices and the nutritional value, microbiology and fermentation profiles of WPCS from dairy farms in the south of the state of Minas Gerais, Brazil. Silages from 54 dairy farms located in nine cities were sampled and a questionnaire was distributed for completion to describe the herd profile and ensilage practices. Herd size ranged from 8 to 1,000 lactating cows, and 52 % of the farms had fewer than 50 cows in lactation. The majority of the farms cultivated hard endosperm texture hybrids (52 %) and used pull-type forage harvesters (87 %). Double-sided plastic was the most used form of silo...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aerobic deterioration; Ensilaging practices; Dairy farming; Lactic acid bacteria.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000200401
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Antibacterial activity of lactic cultures isolated of Italian salami BJM
Maciel,Janeeyre Ferreira; Teixeira,Magdala Alencar; Moraes,Célia Alencar de; Gomide,Lúcio Alberto de Miranda.
Lactic acid bacteria were isolated in the MRS, D-MRS and M17 modified media, during the processing of Italian salami, obtained from two processing plants, in the State of Paraná. The 484 isolates were tested for their antibacterial activity against Listeria monocytogenes (Microbiology Laboratory-UFV), Staphylococcus aureus (ATCC 25923), Salmonella enteritidis (ATCC 13076) and Escherichia coli (ATCC 25922), by a deferred method. One hundred and fifteen isolates inhibited at least two of the pathogens. The isolates presented larger inhibition against L. monocytogenes. The 45 isolates with antagonistic action on the four indicator bacteria were identified by the system Biolog. Lactobacillus bifermentans prevailed among the isolates identified in MRS medium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Italian salami; Lactic acid bacteria; Antibacterial actvity.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500041
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Antibiotic resistance in lactic acid bacteria isolated from some pharmaceutical and dairy products BJM
Gad,Gamal Fadl M.; Abdel-Hamid,Ahmed M.; Farag,Zeinab Shawky H..
A total of 244 lactic acid bacteria (LAB) strains were isolated from 180 dairy and pharmaceutical products that were collected from different areas in Minia governorate, Egypt. LAB were identified phenotypically on basis of morphological, physiological and biochemical characteristics. Lactobacillus isolates were further confirmed using PCR-based assay. By combination of phenotypic with molecular identification Lactobacillus spp. were found to be the dominant genus (138, 76.7%) followed by Streptococcus spp. (65, 36.1%) and Lactococcus spp. (27, 15%). Some contaminant organisms such as (Staphylococcus spp., Escherichia coli, Salmonella spp., mould and yeast) were isolated from the collected dairy samples but pharmaceutical products were free of such...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactic acid bacteria; Phenotypic and molecular identification; PCR assay; Antibiotic resistance genes.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100005
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Antilisterial activity of lactic acid bacteria isolated from vacuum-packaged brazilian meat and meat products BJM
Martinis,Elaine C.P. De; Públio,Márcia R.P.; Santarosa,Priscila R.; Freitas,Flávia Z..
Twenty samples of Brazilian meat and meat products were screened by the agar overlay method for bacteriocin-producing lactic acid bacteria, using Lactobacillus sake ATCC 15521 as indicator strain. Based on Gram staining, KOH reaction, catalase test and fermentation of 49 carbohydrates (API 50 CH), three out of seven isolates with confirmed antagonist properties were identified as Lactobacillus curvatus, one as Leuconostoc mesenteroides and one as Leuconostoc sp. Two isolates could not be properly identified using these tests. The inhibitors produced by these strains were sensitive to proteases. Inhibition due to lytic bacteriophages was ruled out, so the isolates were classified as bacteriocin-producing lactic acid bacteria. Four of them presented...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactic acid bacteria; Meat; Bacteriocins; Biopreservation; Listeria monocytogenes.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100008
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Antioxidant activity of essential oils from condiment plants and their effect on lactic cultures and pathogenic bacteria Ciência Rural
Farias,Paula Karoline Soares; Silva,Júlio César Rodrigues Lopes; Souza,Cintya Neves de; Fonseca,Francine Souza Alves da; Brandi,Igor Viana; Martins,Ernane Ronie; Azevedo,Alcinei Mistico; Almeida,Anna Christina de.
ABSTRACT: Studies about preservative and antioxidant activity of essential oils have been encouraged in recent years, given their importance to food industry. The aim of the current study was to evaluate the antioxidant properties and antimicrobial activity of essential oils deriving from Syzygium aromaticum, Cymbopogon citratus and Lippia alba against lactic and pathogenic bacteria responsible for food-borne diseases. Essential oil antibacterial activity was assessed through disc diffusion and macrodilution tests conducted in a mixed lactic culture of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus (YF-L903) and of Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538), Salmonella enterica (ATCC 6017) strains. Based on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Capim cidreira; Clove; Lemongrass; Lactic acid bacteria; Probiotics.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000200450
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Applications of protective cultures, bacteriocins and bacteriophages in fresh seafood and seafood products ArchiMer
Pilet, Marie-france; Leroi, Francoise.
Microbial seafood-borne disease represents 10 to 20% of the total food-borne outbreaks. Most of them are from bacterial origin and involve seafood products that have been contaminated by pathogenic Vibrio, Listeria monocytogenes and histaminogen bacteria. On the other hand, seafood products are very sensitive to the development of spoiling bacteria producing off-odours. Pathogenic and spoiling microflora are not always reduced or limited by the processing steps that are currently used in these foodstuffs, and the interest for alternative techniques such as bioprotection to improve quality and safety of seafood has increased in the last years. Among the microbial flora of lightly preserved seafood products, lactic acid bacteria usually become dominant...
Tipo: Text Palavras-chave: Biopreservation of seafood; Seafood safety and spoilage; Lactic acid bacteria; Sensory quality; Listeria monocytogenes.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00030/14138/11384.pdf
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Autochthonous probiotic mixture improves biometrical parameters of larvae of Piaractus mesopotamicus (Caracidae, Characiforme, Teleostei) Ciência Rural
Guidoli,Marcos Gabriel; Mendoza,Jorge Arnaldo; Falcón,Sofia Lizardo; Boehringer,Silvia Irene; Sánchez,Sebastián; Macías,María Elena Fátima Nader.
ABSTRACT: Probiotics are a novel alternative to antibiotics as growth factors. Previously, our group isolated, selected and tested in vivo, eight autochthonous strains. They showed no significant effects when administered individually. However, the best doses, stages and ways of administration were combined in a multi strain formula. The aim of this research was to evaluate the effect of this probiotic product on the growth and survival of Piaractus mesopotamicus larvae. The administration was implemented during egg incubation and endogenous feeding period (5), during larvae exogenous feeding period (10) and all along the experiment (15). A group without microorganisms was used as control. The probiotic generates significant increments of mean weight and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactic acid bacteria; Bacillus; Pacú; Larviculture.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700652
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Bacaba beverage produced by Umutina Brazilian Amerindians: Microbiological and chemical characterization BJM
Puerari,Cláudia; Magalhães-Guedes,Karina Teixeira; Schwan,Rosane Freitas.
Bacaba chicha is a beverage prepared by the indigenous Umutina people from the bacaba fruit (Oenocarpus bacaba), a purple berry that is rich in fat and carbohydrates, as well as a source of phenolic compounds. In this study, samples of bacaba chicha beverage were collected, and the microbial community was assessed using culture-dependent and -independent techniques. The nutritional composition and metabolite profiles were analyzed, and species belonging to lactic acid bacteria (LAB) and yeasts were detected. The LAB group detected by culture-dependent analysis included Enterococcus hormaechei and Leuconostoc lactis. Polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) detected additional Propionibacterium avidum, Acetobacter...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Non-alcoholic beverage; Indigenous beverage; Lactic acid bacteria; Yeasts; Bacaba.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401207
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Bacteriocins from lactic acid bacteria: purification, properties and use as biopreservatives BABT
Parada,José Luis; Caron,Carolina Ricoy; Medeiros,Adriane Bianchi P.; Soccol,Carlos Ricardo.
Biopreservation systems in foods are of increasing interest for industry and consumers. Bacteriocinogenic lactic acid bacteria and/or their isolated bacteriocins are considered safe additives (GRAS), useful to control the frequent development of pathogens and spoiling microorganisms in foods and feed. The spreading of bacterial antibiotic resistance and the demand for products with fewer chemicals create the necessity of exploring new alternatives, in order to reduce the abusive use of therapeutic antibiotics. In this context, bacteriocins are indicated to prevent the growth of undesirable bacteria in a food-grade and more natural way, which is convenient for health and accepted by the community. According to their properties, structure, molecular weight...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactic acid bacteria; Bacteriocins; Purification; Food preservation.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300018
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Biological characteristics and probiotic effect of Leuconostoc lactis strain isolated from the intestine of black porgy fish BJM
Zhang,Wei; Liu,Mingqi; Dai,Xianjun.
A strain of lactic acid bacteria, Leuconostoc lactis, was isolated from the intestinal tract of black porgy, Sparus macrocephalus, and identified by conventional biochemical characteristics and 16S rDNA gene sequence analysis. The isolated strain had the ability of bile tolerance and resistance to low pH, and survived well in the trypsinase and pepsin solution. But the highly concentrated dose of trypsinase and pepsin affect the viability of the isolated strain. The isolate was resistant to several antibiotics, including Cephalothin, Ceftriaxone, Imipenem and Tobramycin. The isolate could autoaggregate itself and coaggregate with other bacteria in vitro. The autoaggregation percentage increased to 23.29% after 20 h of incubation. The percentage of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Black porgy; Leuconostoc lactis; Lactic acid bacteria; Biological characteristic; Probiotic.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300004
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Caracterización de cultivos iniciadores en productos cárnicos. Parte 1 OceanDocs
Beldarraín, T.; Cepero, Y.; Bruselas, A.; Santos, R.; Ramos, M.; Moya, Y.; Núñez, M.; Vergara, N..
En este trabajo se caracterizaron tecnológicamente, a escala de laboratorio, 5 cultivos iniciadores. Las propiedades tecnológicas evaluadas fueron pH en caldo glucosado, viabilidad del cultivo, tolerancia a elevada concentración de sal, inactivación a 60 ºC por 30 min, entre otras. Todos los cultivos, por sus aptitudes acidificantes y población celular se consideraron aptos para su empleo industrial y se pudieron categorizar como productores de baja y alta acidez.
Tipo: Journal Contribution Palavras-chave: Bacteria; Starters; Lactic acid bacteria; Microbial starters; Media production; Meat products.
Ano: 2008 URL: http://hdl.handle.net/1834/4896
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Changes in the microbiological and physicochemical characteristics of Serrano cheese during manufacture and ripening BJM
Souza,Claucia Fernanda Volken de; Dalla  Rosa,Tiziano; Ayub,Marco Antônio Zachia.
Changes in the microbial flora present in six Serrano cheese batches (3 ripened in Summer and 3 in Winter) were studied during production and ripening, to microbiologically characterize this cheese, which is traditionally manufactured by farmers in the south of Brazil, using raw cow milk, without the addition of starter cultures. The levels of several microbial groups were enumerated in the milk, curd and different stages of the 60-day long ripening period. Values of some physicochemical parameters, such as pH, titratable acidity, water activity, moisture and NaCl content were also determined. The correlation coefficients between these parameters and the microbial groups throughout ripening were determined. The influence of the season of the year was also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Serrano cheese; Cheese ripening; Food microbiology; Lactic acid bacteria.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300016
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Characterization of Lactobacillus from Algerian goat's milk based on phenotypic, 16S rDNA sequencing and their technological properties BJM
Marroki,Ahmed; Zúñiga,Manuel; Kihal,Mabrouk; Pérez-Martínez,Gaspar.
Nineteen strains of Lactobacillus isolated from goat's milk from farms in north-west of Algeria were characterized. Isolates were identified by phenotypic, physiological and genotypic methods and some of their important technological properties were studied. Phenotypic characterization was carried out by studying physiological, morphological characteristics and carbohydrate fermentation patterns using API 50 CHL system. Isolates were also characterized by partial 16S rDNA sequencing. Results obtained with phenotypic methods were correlated with the genotypic characterization and 13 isolates were identified as L. plantarum, two isolates as L. rhamnosus and one isolate as L. fermentum. Three isolates identified as L. plantarum by phenotypic characterization...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactic acid bacteria; Lactobacillus; Identification; Goat's milk; Technological properties; Algeria.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100020
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Chemical and microbiological evaluation of ensiled sugar cane with different additives BJM
Bravo-Martins,Claudia Eugênia Castro; Carneiro,Heloisa; Castro-Gómez,Raúl Jorge Hernán; Figueiredo,Henrique César Pereira; Schwan,Rosane Freitas.
Sugar cane silage has a potential for animal feeding, but uncontrolled growth of undesirable microorganisms may cause nutritional losses and affect the animal productivity and health. The objective of this work was to evaluate the microbiological quality and chemical composition of ensiled sugar cane with and without nutritional additives after 30 days of fermentation. Yeasts, filamentous fungi and distinct groups of bacteria were enumerated by plate count methods and the chemical analyzes comprised dry matter, crude protein, fiber content, lignin, and pH. Facultative aerobic bacteria and filamentous fungi were not detected during the fermentative process in any of the treatments. The number of yeasts in five varieties of sugar cane silage without...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sugar cane silage; Fermentation; Yeasts; Lactic acid bacteria.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400018
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Chemical inhibition of the contaminant Lactobacillus fermentum from distilleries producing fuel bioethanol BABT
Oliva Neto,Pedro de; Lima,Fabíola Aliaga de; Silva,Ketrin Cristina da; Silva,Douglas Fernandes da; Carvalho,Ana Flavia Azevedo; Santos,Catarina dos.
The purpose of this study was to determine the Minimum Inhibitory Concentration (MIC) of pure or mixed chemicals for Saccharomyces cerevisiae and Lactobacillus fermentum in the samples isolated from distilleries with serious bacterial contamination problems. The biocides, which showed the best results were: 3,4,4' trichlorocarbanilide (TCC), tested at pH 4.0 (MIC = 3.12 mg/l), TCC with benzethonium chloride (CBe) at pH 6.0 (MIC = 3.12 mg/l) and TCC mixed with benzalkonium chloride (CBa) at pH 6.0 (MIC = 1.53 mg /l). If CBa was used in sugar cane milling in 1:1 ratio with TCC, a 8 times reduction of CBa was possible. This formulation also should be tested in fermentation steps since it was more difficult for the bacterium to develop resistance to biocide....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethanol; Lactic acid bacteria; Saccharomyces cerevisiae; Lactobacillus fermentum.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300019
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Comparison of phenotypic and molecular tests to identify lactic acid bacteria BJM
Moraes,Paula Mendonça; Perin,Luana Martins; Silva Júnior,Abelardo; Nero,Luís Augusto.
Twenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic approach is necessary for proper LAB identification, being the molecular analyzes the most reliable.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Lactic acid bacteria; Identification; 16sDNA; PCR; Biolog; API.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100015
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Determining the minimum ripening time of artisanal Minas cheese, a traditional Brazilian cheese BJM
Martins,José M.; Galinari,Éder; Pimentel-Filho,Natan J.; Ribeiro Jr,José I.; Furtado,Mauro M.; Ferreira,Célia L.L.F..
Physical, physicochemical, and microbiological changes were monitored in 256 samples of artisanal Minas cheese from eight producers from Serro region (Minas Gerais, Brazil) for 64 days of ripening to determine the minimum ripening time for the cheese to reach the safe microbiological limits established by Brazilian legislation. The cheeses were produced between dry season (April–September) and rainy season (October–March); 128 cheeses were ripened at room temperature (25 ± 4 °C), and 128 were ripened under refrigeration (8 ± 1 °C), as a control. No Listeria monocytogenes was found, but one cheese under refrigeration had Salmonella at first 15 days of ripening. However, after 22 days, the pathogen was not detected. Seventeen days was the minimum ripening...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ripening; Artisanal Minas cheese; Serro; Pathogen; Lactic acid bacteria.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100219
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Development of microorganisms during storage of wet brewery waste under aerobic and anaerobic conditions R. Bras. Zootec.
Souza,Leiliane Cristine de; Zambom,Maximiliane Alavarse; Pozza,Magali Soares dos Santos; Neres,Marcela Abbado; Radis,Ana Cláudia; Borsatti,Liliane; Castagnara,Deise Dalazen; Gundt,Simoni.
This research study was conducted to evaluate, by means of microbiological analyses, the preservation of wet brewery waste stored under aerobic and anaerobic conditions, regarding the development of filamentous fungi, yeasts and lactic-acid bacteria. The following treatments were used: untreated brewery waste, pre-dried brewery waste silage, brewery waste silage with effluent drainage and BW silage without effluent drainage. Silos made of PVC and equipped with Bunsen valves to allow gases to escape were used. Dry matter (DM), crude protein (CP), temperature (°C) and pH in the untreated BW, in the stored brewery waste and in the brewery waste silage upon silo opening, after 60 days of ensilage were analyzed. A completely randomized design was used. The data...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Filamentous fungi; Lactic acid bacteria; Silage; Yeasts.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000100027
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Dietary Lactic Acid Bacteria Modulate Yolk Components and Cholesterol Metabolism by Hmgr Pathway in Laying Hens Rev. Bras. Ciênc. Avic.
Deng,Q; Shi,H; Luo,Y; Liu,N; Deng,X.
ABSTRACT This study aimed to investigate the effect of dietary lactic acid bacteria (LAB) on egg production, yolk components, cholesterol metabolism, and enterohepatic circulation of bile acids in hens. Four treatment diets included a control and LAB added at 3 × 105 (low), 3 × 107 (medium), or 3 × 109 (high) cfu/kg. The treatment LAB contained equal amounts of Lactobacillus acidophilus, Lactobacillus plantarum, and Enterococcus faecium. Results showed that high LAB increased (p<0.05) laying rate, egg mass, and yolk phospholipid, but decreased (p<0.05) yolk triglyceride and phosvitin. Diets with LAB decreased (p<0.05) yolk and serum cholesterol content, and serum bile acid by 9.3 to 39.9%. In liver, high LAB downregulated (p<0.05) mRNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cholesterol metabolism; HMGR pathway; Lactic acid bacteria; Laying hens; Yolk components.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000300303
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